Used as a synergistic antibacterial agent in veterinary medicine, particularly in combination with sulfonamides to treat bacterial infections in livestock and poultry. It enhances the efficacy of sulfonamides by inhibiting bacterial dihydrofolate reductase, an enzyme involved in folate synthesis. Commonly applied in the prevention and treatment of respiratory, gastrointestinal, and urinary tract infections in animals. Its use supports improved growth performance and disease control in intensive farming systems. Not approved for use in animals producing milk for human consumption due to residue concerns.