Puromycin dihydrochloride
10mM in DMSO
- Product Code: 223982
CAS:
58-58-2
| Molecular Weight: | 544.43 g./mol | Molecular Formula: | C₂₂H₂₉N₇O₅·₂HCl |
|---|---|---|---|
| EC Number: | 200-387-8 | MDL Number: | MFCD00012691 |
| Melting Point: | Boiling Point: | ||
| Density: | Storage Condition: | -20°C |
Product Description:
Puromycin dihydrochloride is widely used as a selection agent in cell culture systems. It effectively kills eukaryotic cells that do not carry resistance genes, making it ideal for selecting genetically modified cells. It is commonly used in stable transfection experiments where cells are engineered to express puromycin N-acetyl-transferase, an enzyme that confers resistance.
It is also used in protein synthesis studies because it acts as an analog of the aminoacyl-tRNA molecule, causing premature chain termination during translation. This property makes it valuable in research involving gene expression and ribosome function.
In addition, puromycin is used in the generation of knockout and knockin cell lines via CRISPR/Cas9 or viral transduction, where rapid selection of transfected or transduced cells is required. Its fast action typically results in cell death within 1–3 days, allowing for quick validation of transfection efficiency and selection of pure cell populations.
Sizes / Availability / Pricing:
| Size | Availability | Price | Quantity |
|---|---|---|---|
| 1ml | 10-20 days | ฿3,440.00 |
+
-
|
Puromycin dihydrochloride
Puromycin dihydrochloride is widely used as a selection agent in cell culture systems. It effectively kills eukaryotic cells that do not carry resistance genes, making it ideal for selecting genetically modified cells. It is commonly used in stable transfection experiments where cells are engineered to express puromycin N-acetyl-transferase, an enzyme that confers resistance.
It is also used in protein synthesis studies because it acts as an analog of the aminoacyl-tRNA molecule, causing premature chain termination during translation. This property makes it valuable in research involving gene expression and ribosome function.
In addition, puromycin is used in the generation of knockout and knockin cell lines via CRISPR/Cas9 or viral transduction, where rapid selection of transfected or transduced cells is required. Its fast action typically results in cell death within 1–3 days, allowing for quick validation of transfection efficiency and selection of pure cell populations.
| Mechanism | - |
| Appearance | - |
| Longevity | - |
| Strength | - |
| Storage | - |
| Shelf Life | - |
| Allergen(s) | - |
| Dosage (Range) | - |
| Recommended Dosage | - |
| Dosage (Per Day) | - |
| Recommended Dosage (Per Day) | - |
| Mix Method | - |
| Heat Resistance | - |
| Stable in pH range | - |
| Solubility | - |
| Product Types | - |
| INCI | - |
Purchase History for
Loading purchase history...
Cart
No products
Subtotal:
฿0.00
฿0.00
Total :