Lysocin is widely used in microbiome research due to its potent antimicrobial activity, particularly against Gram-positive bacteria. It enables selective modulation of microbial communities by targeting specific bacterial populations without disrupting the overall diversity of complex microbiota. This makes it a valuable tool for studying host-microbe interactions, bacterial competition, and the functional role of certain taxa within a microbiome. Its high specificity and purity—being free of DNA contaminants—ensure reliable and reproducible results in sensitive applications such as metagenomic and transcriptomic studies. Lysocin is also employed in the development of infection models, where controlled elimination of specific pathogens helps assess microbial resilience and community dynamics. Additionally, lysocin supports the investigation of antibiotic effects on microbiome composition, offering insights into dysbiosis, resistance mechanisms, and potential therapeutic interventions. Its suitability for in vitro and in vivo systems enhances its utility across diverse experimental setups in microbiology and immunology research.