Gentamicin is widely used as an antibiotic to treat various bacterial infections. It is particularly effective against Gram-negative bacteria, including Pseudomonas, Escherichia coli, and Klebsiella species. Common applications include treating severe infections like sepsis, respiratory tract infections, urinary tract infections, and infections of the skin, bones, and soft tissues. It is also used in combination with other antibiotics to enhance its efficacy. In ophthalmology, gentamicin is applied as eye drops or ointments to treat bacterial eye infections. Additionally, it is used in veterinary medicine to manage infections in animals. Due to its potential for causing side effects like kidney damage and hearing loss, it is typically reserved for serious infections and administered under medical supervision.