Used in biomedical research to detect the activated form of ERK1 and ERK2 proteins in cells. This antibody specifically recognizes ERK1/2 when phosphorylated at Thr202 and Tyr204, which indicates their active state in the MAP kinase signaling pathway. It is commonly applied in Western blotting, immunocytochemistry, and flow cytometry to study cellular responses to growth factors, cytokines, and stress signals. Researchers use it to investigate processes like cell proliferation, differentiation, and apoptosis, as well as in cancer research to assess pathway activation in tumor samples.