6-Fluoro-4-hydroxycoumarin is primarily utilized in the field of organic synthesis and medicinal chemistry. It serves as a key intermediate in the development of various pharmaceutical compounds, particularly those with anticoagulant properties. This chemical is often employed in the synthesis of novel coumarin derivatives, which are explored for their potential therapeutic applications, including anti-inflammatory, anticancer, and antimicrobial activities. Additionally, it is used in research to study enzyme inhibition mechanisms, especially those involving vitamin K antagonists. Its fluorescence properties also make it valuable in biochemical assays and as a probe in cellular imaging studies.