ANA-12 is a selective antagonist of the TrkB receptor, which plays a key role in the signaling of brain-derived neurotrophic factor (BDNF). Due to its ability to modulate BDNF pathways, ANA-12 is widely used in neuroscience research to study mood disorders such as depression and anxiety. It helps researchers investigate the role of neurotrophic signaling in behavioral models, particularly in stress-induced models of depression. Studies have shown that ANA-12 can block the behavioral effects of BDNF, making it a valuable tool for understanding the mechanisms behind antidepressant efficacy. It is also used in research on neuroplasticity, learning, and memory, as well as in studies exploring addiction and neurodegenerative diseases. Its application remains primarily experimental, serving as a pharmacological probe in preclinical models.