Tuberospironine A exhibits significant antifungal activity, particularly against plant pathogenic fungi, making it a candidate for use in agricultural fungicides. It has shown inhibitory effects on spore germination and mycelial growth in fungi such as Fusarium and Botrytis species, which are responsible for major crop diseases. Due to its natural origin and specific mode of action, it is being studied for development as a bio-based pesticide to reduce reliance on synthetic chemicals in sustainable farming. Additionally, its unique spirocyclic structure contributes to interest in pharmaceutical research, where it serves as a lead compound for designing new bioactive agents.