Thalifendine is primarily used in research settings for its bioactive properties, particularly in studies related to enzyme inhibition and cellular signaling pathways. It has shown potential as an antimicrobial agent, with activity observed against certain Gram-positive bacteria. Its structure allows for interaction with specific protein targets, making it a candidate for development in anti-inflammatory and anticancer research. Due to its natural origin and structural complexity, it is also used as a reference compound in phytochemical analysis and drug discovery screening programs. However, it remains under investigation and is not currently used in clinical medicine.