Boromycin is primarily used as an antibiotic, showing strong activity against Gram-positive bacteria. It has been studied for its ability to inhibit bacterial protein synthesis by targeting the ribosome, making it effective in controlling certain bacterial infections. Due to its unique structure containing boron, it also serves as a model compound in research for understanding boron’s role in bioactive natural products. Additionally, boromycin exhibits antifungal and antiparasitic properties, with some studies exploring its potential in treating malaria and other protozoal infections. Its ionophoric properties allow it to transport potassium ions across membranes, which contributes to its antimicrobial mechanism.