Pyrazinoic acid hydrazide is primarily used in the field of medicinal chemistry and pharmaceutical research. It serves as a key intermediate in the synthesis of antitubercular agents, particularly in the development of drugs targeting Mycobacterium tuberculosis. Its hydrazide functional group makes it a valuable building block for creating derivatives with enhanced biological activity. Additionally, it is employed in studies focusing on the mechanism of action of pyrazinamide, a first-line antituberculosis drug, to better understand resistance patterns and improve therapeutic efficacy. Researchers also explore its potential in designing novel compounds with antimicrobial properties for treating drug-resistant infections.