Bufotalin is primarily used in research settings to study its effects on cellular processes, particularly its impact on cancer cells. It has shown potential in inducing apoptosis, making it a subject of interest in oncology for developing new cancer therapies. Additionally, its cardiotoxic properties are studied to understand its mechanisms and explore potential therapeutic applications or antidotes. In traditional medicine, it has been used in small, controlled doses for its purported medicinal benefits, though its toxicity requires careful handling and further scientific validation.