Used as a fluorescent substrate for detecting caspase-3 and caspase-7 activity in apoptosis assays. The compound becomes fluorescent upon cleavage by these enzymes, allowing real-time monitoring of programmed cell death in live cells or cell lysates. Commonly applied in high-throughput screening for drug discovery, toxicology studies, and research on cellular death pathways. Its Z-DEVD sequence is specifically recognized by caspase-3/7, ensuring high selectivity. The R110 (rhodamine 110) moiety provides a strong fluorescent signal after enzymatic cleavage, making it suitable for fluorometric detection methods.