Used in biomedical research to quantify levels of Apoptosis Inducing Factor 1 (AIF-1) in mouse samples. Helps investigate cell death mechanisms, particularly in studies related to neurodegenerative diseases, ischemia-reperfusion injury, and mitochondrial dysfunction. Enables researchers to assess AIF-1 release from mitochondria into the cytoplasm and nucleus, a key step in caspase-independent apoptosis. Widely applied in preclinical studies to evaluate therapeutic interventions targeting apoptosis pathways. Provides a reliable, sensitive, and specific method for high-throughput analysis of AIF-1 in tissue homogenates and cell lysates.