Used in biomedical research to quantify levels of the BH3 domain apoptosis-induced protein (Bid) in rat samples. Helps study apoptosis pathways, particularly in liver injury, neurodegenerative diseases, and ischemia-reperfusion damage. Enables researchers to assess mitochondrial-dependent cell death mechanisms and evaluate potential therapeutic interventions targeting apoptosis regulation. Commonly applied in preclinical studies using rat models to understand disease progression and drug effects.