PETCM is primarily utilized in biochemical research as a potent inhibitor of the mitochondrial permeability transition pore (MPTP). Its application is crucial in studies focused on understanding cellular mechanisms related to apoptosis and necrosis, particularly in the context of ischemia-reperfusion injury. Researchers employ PETCM to explore protective strategies against cell death in various pathological conditions, including heart attacks and neurodegenerative diseases. Additionally, it serves as a valuable tool in pharmacological studies aimed at developing new therapeutic agents that target mitochondrial dysfunction.