This chemical is widely used in bioconjugation and labeling applications due to its unique functional groups. The biotin moiety allows for strong binding to avidin or streptavidin, making it valuable in affinity purification, detection, and imaging studies. The PEG4 spacer enhances solubility and reduces steric hindrance, improving accessibility in biological systems. The amino-t-Bu-DADPS group provides stability and protection during synthetic processes, while the C6-azide enables click chemistry reactions, particularly with alkyne-containing molecules, for site-specific modifications. This compound is particularly useful in drug delivery systems, diagnostic assays, and the development of targeted therapeutics, where precise labeling and efficient conjugation are critical.