This chemical is widely used in bioconjugation and click chemistry applications due to its dual functional groups. The thiol (SH) group allows for covalent attachment to cysteine residues or other thiol-containing molecules, while the azide (N3) group participates in copper-catalyzed azide-alkyne cycloaddition (CuAAC) reactions. Its polyethylene glycol (PEG) spacer enhances solubility and reduces steric hindrance, making it ideal for modifying biomolecules like proteins, peptides, and antibodies. It is particularly useful in drug delivery systems, surface functionalization, and the development of targeted therapeutics. Additionally, it plays a role in creating hydrogels and other biomaterials for tissue engineering and regenerative medicine.