This chemical is widely used in bioconjugation and labeling applications due to its dual functional groups. The azide group allows for click chemistry reactions, particularly with alkynes, enabling efficient and selective conjugation to biomolecules. The maleimide group reacts with thiols, making it suitable for attaching to cysteine residues in proteins or peptides. The Cy5 fluorophore provides strong fluorescence in the red region, making it ideal for imaging and tracking in biological systems. The PEG spacers enhance solubility and reduce steric hindrance, improving the efficiency of conjugation and the stability of the resulting constructs. It is commonly employed in fluorescence microscopy, flow cytometry, and in vivo imaging studies to visualize and monitor cellular processes.