This compound is primarily used in bioconjugation and labeling applications due to its unique combination of functional groups. The fluorescein moiety allows for fluorescent detection, making it valuable in imaging and tracking biological molecules in research. The thiourea group enhances stability and binding interactions, while the PEG2 spacer improves solubility and reduces steric hindrance in biological systems. The azide group enables click chemistry reactions, particularly with alkyne-containing molecules, facilitating the attachment of the compound to proteins, peptides, or other biomolecules. It is widely utilized in fluorescence microscopy, flow cytometry, and other analytical techniques to study cellular processes, protein interactions, and molecular dynamics. Additionally, its properties make it suitable for developing diagnostic probes and therapeutic agents in biomedical research.