This compound is widely used in bioconjugation and chemical biology due to its ability to link biomolecules, such as proteins, peptides, and antibodies, with other functional groups or surfaces. The azide (N3) group allows for efficient click chemistry reactions, particularly with alkynes, enabling the formation of stable triazole linkages. The PEG (polyethylene glycol) spacer provides flexibility, solubility, and reduced steric hindrance, making it ideal for creating biocompatible conjugates. The carboxyl (COOH) group offers further functionalization opportunities, such as coupling with amines via EDC/NHS chemistry. It is commonly applied in drug delivery systems, diagnostic probes, and surface modification of nanomaterials for biomedical research and therapeutic applications.