Used in bioconjugation and labeling applications, particularly in click chemistry reactions. The azide group readily participates in copper-catalyzed azide-alkyne cycloaddition (CuAAC), enabling efficient attachment to alkyne-functionalized biomolecules such as proteins, peptides, or nucleic acids. Commonly employed in probe development for fluorescence imaging, affinity tagging, and drug discovery. Its aliphatic chain provides flexibility and spacing, reducing steric hindrance in conjugated systems. Also utilized in surface modification and immobilization strategies on solid supports for biochemical assays.