Used in bioconjugation and affinity purification workflows, this reagent enables selective labeling and capture of biomolecules. The azide group participates in copper-catalyzed or strain-promoted azide-alkyne cycloaddition (click chemistry), allowing specific attachment to alkyne-modified proteins, nucleic acids, or cell surface markers. The disulfide (Ss) linker provides a cleavable site under reducing conditions, facilitating release of captured targets during downstream analysis. Biotin enables strong interaction with streptavidin-based matrices or detection reagents, making it ideal for pull-down assays, target enrichment, or visualization in complex biological samples. Commonly applied in proteomics, chemical biology, and drug discovery for reversible tagging and isolation of biomolecules.