Used as a key reagent in click chemistry for bioconjugation and labeling applications. Its azide group readily participates in copper-catalyzed azide-alkyne cycloaddition (CuAAC) reactions, enabling efficient attachment to biomolecules such as proteins, nucleic acids, and antibodies. The acetyl and benziodazolone moieties enhance reactivity and stability during conjugation, making it suitable for developing diagnostic probes and targeted therapeutics. Also employed in activity-based protein profiling (ABPP) to study enzyme function in complex biological systems. Its ability to react selectively under mild conditions allows for labeling in live cells with minimal interference to native processes.