Widely used in bioconjugation and labeling applications, this compound is particularly valuable in fluorescence-based imaging and detection techniques. Its azide group enables efficient click chemistry reactions, especially copper-catalyzed azide-alkyne cycloaddition (CuAAC), allowing selective attachment to biomolecules such as proteins, nucleic acids, or small molecules bearing alkyne groups. The carboxy functionality provides a handle for further amide coupling, enabling stable linkage to amine-containing targets. Due to its strong fluorescence and high quantum yield, it is employed in cellular imaging, flow cytometry, and immunoassays. Its red-shifted emission minimizes background autofluorescence in biological samples, making it ideal for multiplex labeling when combined with other fluorophores. Commonly used in research settings for tracking biomolecular interactions and localization in live or fixed cells.