This compound is primarily utilized in bioconjugation and chemical biology research. It serves as a linker molecule, enabling the attachment of biomolecules, such as proteins or peptides, to other functional groups or surfaces. The pyrene moiety allows for strong non-covalent interactions, particularly with carbon-based materials like graphene or carbon nanotubes, making it useful in nanotechnology and material science applications. The PEG4 spacer provides flexibility and solubility, enhancing the stability of the conjugate in aqueous environments. The azide group facilitates click chemistry reactions, particularly copper-catalyzed azide-alkyne cycloaddition (CuAAC), which is widely employed for labeling, imaging, and drug delivery systems. This compound is also valuable in the development of biosensors, where its properties enable precise and efficient molecular recognition and signal transduction.