Pyrene azide 3 is widely used in bioorthogonal chemistry for labeling and imaging biomolecules. Its azide group enables selective reactions with alkyne-functionalized probes via click chemistry, making it valuable for studying cellular processes. The pyrene moiety provides strong fluorescence, allowing for sensitive detection in fluorescence microscopy and spectroscopy. It is also employed in materials science for surface modification and functionalization of polymers, nanoparticles, and other substrates. Additionally, it serves as a crosslinking agent in the development of advanced materials with tailored properties. Its dual functionality makes it a versatile tool in both biological and chemical research.