This chemical is widely used in bioconjugation and crosslinking applications due to its dual NHS ester groups, which react efficiently with primary amines to form stable amide bonds. The azide group enables click chemistry, particularly in copper-catalyzed azide-alkyne cycloaddition (CuAAC), making it valuable for labeling, modifying, and linking biomolecules such as proteins, peptides, and nucleic acids. Its PEG3 spacer enhances solubility and reduces steric hindrance, improving reaction efficiency in complex biological systems. It is commonly employed in drug delivery systems, antibody-drug conjugates, and the development of biosensors, where precise and stable molecular linkages are essential.