This compound is widely used in bioconjugation chemistry, particularly for labeling biomolecules such as proteins, peptides, and nucleic acids. Its biotin moiety allows for strong affinity binding with streptavidin or avidin, enabling applications in affinity purification, detection, and immobilization. The azide group facilitates click chemistry reactions, such as the copper-catalyzed azide-alkyne cycloaddition (CuAAC), making it a valuable tool for attaching biotin tags to target molecules in a highly specific and efficient manner. It is commonly employed in proteomics, cellular imaging, and drug discovery to study molecular interactions and pathways. Additionally, its use in fluorescence labeling and surface modification enhances its utility in diagnostic assays and biomaterial research.