This compound is primarily utilized in peptide synthesis and bioconjugation processes. It serves as a building block for introducing azide functional groups into peptides, enabling click chemistry reactions. The fluorenylmethoxycarbonyl (Fmoc) group acts as a protective group during solid-phase peptide synthesis, ensuring selective reactions at specific sites. The azide moiety allows for efficient conjugation with alkyne-containing molecules via copper-catalyzed azide-alkyne cycloaddition (CuAAC), a widely used method in bioorthogonal chemistry. This makes it valuable for labeling, modifying, or crosslinking biomolecules in research applications, such as drug development, protein engineering, and biomaterial design. Its use is particularly significant in creating peptide-based probes or therapeutics with tailored functionalities.