2-Azidoacetic acid is primarily utilized in organic synthesis and chemical research. It serves as a key intermediate in the preparation of various azide-containing compounds, which are valuable in click chemistry reactions, particularly the copper-catalyzed azide-alkyne cycloaddition (CuAAC). This reaction is widely employed in bioconjugation, material science, and drug discovery for labeling, crosslinking, and functionalizing molecules. Additionally, 2-azidoacetic acid is used in the synthesis of peptides and other bioactive molecules, where the azido group can be further modified to introduce specific functionalities. Its applications also extend to polymer chemistry, where it aids in the development of advanced materials with tailored properties.