This compound is primarily used in bioconjugation and protein modification processes. It serves as a linker molecule, enabling the attachment of azide-functionalized groups to biomolecules like peptides or proteins. The PEG3 spacer enhances solubility and reduces steric hindrance, making it suitable for applications in drug delivery systems, where controlled release and targeted action are essential. Additionally, it is utilized in click chemistry reactions, particularly copper-catalyzed azide-alkyne cycloaddition (CuAAC), to create stable triazole linkages for labeling or imaging studies in biological research. Its alanine component allows for integration into peptide sequences, facilitating the development of bioactive compounds.