This compound is widely used in bioconjugation and chemical biology due to its reactive azide group and polyethylene glycol (PEG) spacers. The azide group enables click chemistry reactions, particularly copper-catalyzed azide-alkyne cycloaddition (CuAAC), making it valuable for labeling, modifying, or crosslinking biomolecules like proteins, peptides, and nucleic acids. The PEG spacers enhance solubility and reduce steric hindrance, improving the efficiency of conjugation processes. The t-butyl ester group serves as a protective moiety, which can be removed under acidic conditions to expose a carboxylic acid for further functionalization. This compound is particularly useful in drug delivery systems, biopolymer modification, and the development of biocompatible materials. Its design allows for precise control over molecular interactions, making it a versatile tool in research and therapeutic applications.