This chemical is primarily used in bioconjugation and click chemistry applications. It serves as a linker molecule, enabling the attachment of biomolecules, such as proteins, peptides, or nucleic acids, to other entities like nanoparticles, surfaces, or fluorescent dyes. The azide group allows for efficient and selective reactions with alkyne-functionalized compounds through copper-catalyzed azide-alkyne cycloaddition (CuAAC), a widely used click chemistry reaction. The PEG (polyethylene glycol) spacer enhances solubility, reduces steric hindrance, and improves biocompatibility, making it suitable for use in biological and pharmaceutical research. The t-butyl ester group can be deprotected to generate a carboxylic acid, providing further functionalization opportunities. This compound is particularly valuable in drug delivery systems, diagnostic tools, and materials science for creating tailored biomolecular constructs.