This compound is widely utilized in bioconjugation and chemical biology research due to its reactive NHS ester group and azide functionality. The NHS ester enables efficient coupling with primary amines, commonly found in proteins, peptides, and other biomolecules, while the azide group allows for further modification via click chemistry, such as copper-catalyzed azide-alkyne cycloaddition (CuAAC). This dual functionality makes it valuable for labeling, crosslinking, and functionalizing biomolecules in applications like drug delivery, surface modification, and the development of bioassays. Its PEG spacer enhances solubility and reduces steric hindrance, improving the efficiency of reactions in aqueous environments.