UV cleavable biotin-PEG2-azide is widely used in biochemical research for its unique properties. It serves as a versatile tool for labeling and isolating biomolecules. The biotin moiety allows for strong binding to streptavidin or avidin, enabling efficient capture and purification of target molecules. The PEG2 spacer enhances solubility and reduces steric hindrance, improving interaction efficiency. The azide group facilitates click chemistry reactions, particularly copper-catalyzed azide-alkyne cycloaddition (CuAAC), for site-specific conjugation. The UV-cleavable feature provides precise control over the release of captured molecules under UV light, making it ideal for applications like affinity purification, protein-protein interaction studies, and controlled release systems. Its modular design and functionality make it a valuable reagent in proteomics, drug delivery, and molecular biology research.