LtaS-IN-1 is primarily used in research settings to study bacterial cell wall synthesis and its inhibition. It acts as a potent inhibitor of LtaS, an enzyme crucial for the production of lipoteichoic acid in Gram-positive bacteria. By targeting LtaS, this compound helps researchers understand the mechanisms of bacterial survival and resistance, providing insights into potential therapeutic strategies against infections caused by pathogens like Staphylococcus aureus. Its application is significant in the development of novel antibiotics, particularly for combating drug-resistant bacterial strains.