Aspoxicillin is a beta-lactam antibiotic primarily used in research settings due to its structural and functional properties. It exhibits activity against a range of Gram-positive and some Gram-negative bacteria by inhibiting cell wall synthesis. Its main application lies in antimicrobial susceptibility testing, where it helps determine bacterial resistance patterns in clinical and laboratory strains. Due to its stability and specific mode of action, it is also used as a reference compound in the development and calibration of analytical methods for penicillin-class antibiotics. Aspoxicillin is not widely used in clinical therapy but serves as a valuable tool in pharmaceutical research and quality control processes.