α-Muricholic acid is primarily utilized in research focused on bile acid metabolism and its role in digestive health. It serves as a key component in studies investigating the regulation of cholesterol levels and lipid absorption in the body. Additionally, it is employed in the development of therapeutic agents targeting liver and gastrointestinal disorders. Its applications also extend to the study of gut microbiota interactions, as it influences microbial composition and function. This compound is further explored in the formulation of drugs aimed at treating metabolic syndromes and bile acid-related diseases.