Bromobimane is widely used as a fluorescent labeling agent in biochemical and cellular research. It reacts specifically with thiol groups, making it valuable for tagging proteins, peptides, and other biomolecules containing cysteine residues. This property allows researchers to track and visualize these molecules in complex biological systems. It is particularly useful in studying protein structure, dynamics, and interactions, as well as in monitoring redox states within cells. Additionally, its fluorescence properties enable its use in flow cytometry, microscopy, and other imaging techniques to study cellular processes in real-time.