This compound is widely used in bioorthogonal chemistry for labeling and conjugating biomolecules. Its DBCO group enables rapid and selective reactions with azides through strain-promoted alkyne-azide cycloaddition (SPAAC), making it ideal for live-cell imaging and protein modification without interfering with biological processes. The NHS ester group allows for efficient coupling with primary amines, facilitating the attachment to proteins, peptides, or other amine-containing molecules. The disulfide bond (S-S) provides a cleavable linkage, enabling controlled release of conjugated molecules under reducing conditions, which is particularly useful in drug delivery systems and targeted therapies. Its applications span across bioconjugation, antibody-drug conjugates, and the development of diagnostic tools.