Used in bone metabolism research to measure β-isomerized C-terminal telopeptide of type I collagen (β-CTx), a key biomarker for osteoclast-mediated bone resorption. Enables assessment of bone turnover in preclinical studies, particularly in osteoporosis, metabolic bone diseases, and drug development. Commonly applied in rodent models to evaluate the efficacy of anti-resorptive therapies such as bisphosphonates or denosumab analogs. Provides a sensitive and specific quantification method in serum or plasma samples, supporting longitudinal studies without requiring terminal sampling. Widely used in pharmaceutical and academic research for monitoring treatment effects on bone degradation.