Used in bone metabolism research to quantify crosslinked C-telopeptides of type I collagen in biological samples, primarily urine and serum. This measurement serves as a marker for bone resorption, helping assess osteoclast activity. Widely applied in studies of osteoporosis, Paget's disease, and other metabolic bone disorders. Enables evaluation of antiresorptive drug efficacy, such as bisphosphonates and denosumab, in clinical and preclinical settings. Also used in monitoring bone turnover in postmenopausal women, elderly populations, and patients with cancer-related bone metastases. Provides a sensitive and specific tool for high-throughput screening in laboratory research, supporting longitudinal studies of bone health and disease progression.