Used in research to measure zonulin levels in biological samples, helping assess intestinal permeability and gut barrier function. Commonly applied in studies related to autoimmune diseases, inflammatory bowel disease, celiac disease, type 1 diabetes, and obesity. Enables investigation into the role of gut health in chronic conditions by quantifying zonulin, a key regulator of tight junctions in the gut lining. Widely used in both clinical and preclinical research settings.