Used in bone metabolism research to measure bone resorption levels by detecting crosslinked C-telopeptides of type I collagen in biological samples such as serum or urine. Commonly applied in studies of osteoporosis, metabolic bone diseases, and evaluation of anti-resorptive drug efficacy. Provides a sensitive and specific method for monitoring collagen breakdown, helping assess bone turnover rates in clinical and preclinical settings. Widely used in pharmaceutical research and diagnostic laboratories for biomarker analysis.