Used in bone metabolism research to quantify osteocalcin levels in human serum or plasma samples. Helps assess bone formation and turnover in studies related to osteoporosis, metabolic bone diseases, and endocrine disorders. Commonly applied in clinical research and pharmaceutical development to evaluate the effectiveness of treatments affecting bone health. Provides a reliable, sensitive, and specific measurement for the N-terminal mid-fragment of osteocalcin, a key biomarker produced by osteoblasts.