Used in biomedical research to quantify osteoprotegerin levels in rat samples, aiding studies on bone metabolism, osteoporosis, and inflammatory diseases. Enables researchers to assess the role of OPG in bone remodeling, calcium regulation, and its interaction with RANKL in experimental models. Commonly applied in preclinical studies involving arthritis, periodontal disease, and bone loss due to hormonal changes. Provides a reliable, sensitive method for detecting OPG in serum, plasma, and cell culture supernatants, supporting drug development and mechanistic investigations in bone-related disorders.