Used in environmental and toxicological research to detect and quantify vitellogenin (VTG) in biological samples, this ELISA kit helps assess endocrine-disrupting chemical exposure in aquatic organisms. It is particularly applied in studies involving fish and other vertebrates to monitor estrogenic activity caused by pollutants such as pesticides, industrial chemicals, and pharmaceuticals. The kit enables high-throughput analysis in laboratory settings, supporting research on reproductive health, wildlife conservation, and water quality assessment. Due to its specificity for microbial-expressed yolk protein, it is also utilized in aquaculture and biotechnology to study protein expression and hormonal regulation.