Used in bioconjugation processes to link biomolecules such as proteins, peptides, or antibodies with detection reagents or solid supports. The biotin moiety binds strongly to streptavidin or avidin, making it ideal for applications in immunoassays, ELISA, flow cytometry, and diagnostic probe development. The spacer arm provided by the amidoethylacetamide group enhances accessibility and binding efficiency, reducing steric hindrance. Commonly employed in labeling strategies for purification, detection, and immobilization of biomolecules in research and diagnostic settings.