This chemical is widely used in bioconjugation and labeling applications due to its biotin and PEG4 components. The biotin moiety allows for strong and specific binding to avidin or streptavidin, making it valuable in affinity purification, immunoassays, and diagnostic tools. The PEG4 spacers enhance solubility and reduce steric hindrance, improving the efficiency of binding interactions. The t-butyl ester groups provide protection for carboxylic acids, enabling controlled activation and coupling in peptide synthesis or other chemical modifications. It is particularly useful in drug delivery systems, where biotinylation targets specific cells or tissues, and in research applications for tagging biomolecules like proteins or antibodies.